MYO5A antibody (Internal Region)

Catalog No. ABIN571223

Product Details anti-MYO5A Antibody

Target: MYO5A (Myosin VA (MYO5A))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MYO5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: MYO5A

Sequence: ETKQLELDLN

Specificity: This antibody is expected to recognize both reported isoforms (NP_000250.3, NP_001135967.1).

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-ETKQLELDLN

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 200 kDa band observed in lysates of cell line Jurkat (calculated MW of 215 kDa according to NP_000250.3). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, P

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MYO5A

Target: MYO5A (Myosin VA (MYO5A))

Alternative Name: MYO5A (MYO5A Products)

Synonyms: myo5a antibody, MGC53270 antibody, si:dkey-266j9.1 antibody, MYOA antibody, MYO5A antibody, D antibody, Dop antibody, Myh12 antibody, 9630007J19Rik antibody, AI413174 antibody, AI661011 antibody, Dbv antibody, MVa antibody, Myo5 antibody, MyoVA antibody, Sev-1 antibody, d antibody, d-120J antibody, flail antibody, flr antibody, DILUTE antibody, myoxin) antibody, non-muscle antibody, GS1 antibody, MYH12 antibody, MYO5 antibody, MYR12 antibody, myosin VA L homeolog antibody, myosin VAa antibody, myosin VA antibody, myosin va antibody, myo5a.L antibody, myo5aa antibody, MYO5A antibody, PY04789 antibody, Myo5a antibody

Background: MYO5A, myosin VA (heavy chain 12, myoxin), GS1, MYH12, MYO5, MYR12, dilute, myosin V, myosin VA, myosin, heavy polypeptide kinase, myoxin

Gene ID: 4644, 17918, 25017

NCBI Accession: NP_000250, NP_001135967

Pathways: Hormone Transport, Peptide Hormone Metabolism