TRIM2 antibody (AA 758-771)

Catalog No. ABIN605050

Product Details anti-TRIM2 Antibody

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Binding Specificity: AA 758-771

Reactivity: Human, Mouse, Rat, Dog, Cow, Chicken, Bat, Hamster, Horse, Monkey, Rabbit, Xenopus laevis, Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TRIM2 / RNF86. This antibody is expected to recognise both reported isoforms (NP_056086.2, NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish (100%) Pig (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide DSGNHCFKVYRYLQ from the C-terminus of human TRIM2 / RNF86 (NP_056086.2, NP_001123539.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish (100%), Pig (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (6.25 μg/mL), Peptide-ELISA (1:16000), WB (0.5 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 80+85 kDa bands observed in Rat Brain lysates (calculated MW of 81.5 kDa according to Human NP_001123539.1, 84.4 accorindg to Human NP_056086.2 and 83.3 according to Rat NP_001102022.1). Recommended concentration: 0.5-2 μg/mL.. An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TRIM2

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Alternative Name: KIAA0517 / TRIM2 (TRIM2 Products)

Synonyms: RNF86 antibody, mKIAA0517 antibody, narf antibody, trim2 antibody, zgc:113099 antibody, si:dkeyp-75b4.11 antibody, tripartite motif containing 2 antibody, tripartite motif-containing 2 antibody, tripartite motif containing 2a antibody, tripartite motif containing 2 S homeolog antibody, tripartite motif containing 2b antibody, TRIM2 antibody, Trim2 antibody, trim2a antibody, trim2.S antibody, trim2b antibody

Background: Name/Gene ID: TRIM2
Family: Tripartite Motif

Synonyms: TRIM2, KIAA0517, RNF86, RING finger protein 86, Tripartite motif containing 2, Tripartite motif protein 2, Tripartite motif protein TRIM2, Tripartite motif-containing 2

Gene ID: 23321

NCBI Accession: NP_056086, NP_001123539

UniProt: Q9C040