PYGM antibody (C-Term)

Catalog No. ABIN6746291

This anti-PYGM antibody is a Rabbit Polyclonal antibody detecting PYGM in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Rabbit, Sheep, Guinea Pig, Monkey and Pig. This Primary Antibody has been cited in 2+ publications.

Quick Overview for PYGM antibody (C-Term) (ABIN6746291)

Target: PYGM (Phosphorylase, Glycogen, Muscle (PYGM))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Sheep, Guinea Pig, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYGM antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PYGM Antibody

Binding Specificity: C-Term

Specificity: Mouse PYGM

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Pig, Guinea pig (100%) Horse (92%) Sheep, Dog, Bovine (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of mouse Pygm (Q9WUB3, NP_035354). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Rabbit, Pig, Guinea pig (100%), Elephant, Bat, Horse, Armadillo (92%), Sheep, Dog, Bovine (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PYGM antibody (ABIN6746291)

Hertel, Zhou, Hamilton-Dutoit, Junker: "Loss of B cell identity correlates with loss of B cell-specific transcription factors in Hodgkin/Reed-Sternberg cells of classical Hodgkin lymphoma." in: Oncogene, Vol. 21, Issue 32, pp. 4908-20, (2002) (PubMed).

Foss, Reusch, Demel, Lenz, Anagnostopoulos, Hummel, Stein: "Frequent expression of the B-cell-specific activator protein in Reed-Sternberg cells of classical Hodgkin's disease provides further evidence for its B-cell origin." in: Blood, Vol. 94, Issue 9, pp. 3108-13, (1999) (PubMed).

Target Details for PYGM

Target: PYGM (Phosphorylase, Glycogen, Muscle (PYGM))

Alternative Name: PYGM

Background: Name/Gene ID: PYGM

Synonyms: PYGM, GPMM, Myophosphorylase, Phosphorylase, glycogen muscle

Gene ID: 5837

NCBI Accession: NP_035354

UniProt: P11217

Pathways: Cellular Glucan Metabolic Process