ARID5B antibody (AA 1031-1080)

Catalog No. ABIN6746321

The Rabbit Polyclonal anti-ARID5B antibody has been validated for WB. It is suitable to detect ARID5B in samples from Human, Cow, Horse, Rabbit, Rat, Monkey, Hamster and Pig. There is 1 publication available.

Quick Overview for ARID5B antibody (AA 1031-1080) (ABIN6746321)

Target: ARID5B (AT Rich Interactive Domain 5B (MRF1-Like) (ARID5B))

Reactivity: Human, Cow, Horse, Rabbit, Rat, Monkey, Hamster, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID5B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARID5B Antibody

Binding Specificity: AA 1031-1080

Specificity: Human ARID5B

Predicted Reactivity: Percent identity by BLAST analysis: Bovine, Rabbit, Horse (100%) Rat (92%) Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1031-1080 of human ARID5B (Q14865, NP_115575). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Rat, Hamster, Bovine, Rabbit, Horse (100%), Mouse, Bat (92%), Elephant, Pig, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ARID5B antibody (ABIN6746321)

Aspberg, Miura, Bourdoulous, Shimonaka, Heinegârd, Schachner, Ruoslahti, Yamaguchi: "The C-type lectin domains of lecticans, a family of aggregating chondroitin sulfate proteoglycans, bind tenascin-R by protein-protein interactions independent of carbohydrate moiety." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 19, pp. 10116-21, (1997) (PubMed).

Target Details for ARID5B

Target: ARID5B (AT Rich Interactive Domain 5B (MRF1-Like) (ARID5B))

Alternative Name: ARID5B

Background: Name/Gene ID: ARID5B

Synonyms: ARID5B, DESRT, Modulator recognition factor 2, MRF2, MRF-2, MRF1-like protein

Gene ID: 84159

NCBI Accession: NP_115575

UniProt: Q14865

Pathways: Platelet-derived growth Factor Receptor Signaling