RCAS1 antibody (C-Term)

Catalog No. ABIN6746697

This Rabbit Polyclonal antibody specifically detects RCAS1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow, Horse, Guinea Pig, Chicken, Bat, Monkey and Pig. It has been mentioned in 3+ publications

Quick Overview for RCAS1 antibody (C-Term) (ABIN6746697)

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Horse, Guinea Pig, Chicken, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCAS1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RCAS1 Antibody

Binding Specificity: C-Term

Specificity: Human EBAG9

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Chicken (100%) Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human EBAG9 (O00559, NP_004206). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard (100%), Dog, Xenopus, Frog, Catfish, Zebrafish (92%), Pufferfish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-RCAS1 antibody (ABIN6746697)

Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

Zong, Ren, Young, Pypaert, Mu, Birnbaum, Shulman: "AMP kinase is required for mitochondrial biogenesis in skeletal muscle in response to chronic energy deprivation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 15983-7, (2002) (PubMed).

Brocke, Chiang, Wagner, Schulman: "Functional implications of the subunit composition of neuronal CaM kinase II." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22713-22, (1999) (PubMed).

Target Details for RCAS1

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Alternative Name: RCAS1

Background: Name/Gene ID: EBAG9

Synonyms: EBAG9, EB9, PDAF, RCAS1

Gene ID: 9166

NCBI Accession: NP_004206

UniProt: O00559