ADRA1B antibody (AA 388-437)

Catalog No. ABIN6746726

There are 3+ publications for this product available. The Rabbit Polyclonal anti-ADRA1B antibody is suitable to detect ADRA1B in samples from Human, Mouse, Rat, Dog, Cow, Pig, Monkey, Guinea Pig, Rabbit and Hamster. It has been validated for WB.

Quick Overview for ADRA1B antibody (AA 388-437) (ABIN6746726)

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Monkey, Guinea Pig, Rabbit, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRA1B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADRA1B Antibody

Binding Specificity: AA 388-437

Specificity: Human ADRA1B

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Bovine, Rabbit, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa388-437 of human ADRA1B (P35368, NP_000670). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Pig, Opossum, Guinea pig, Platypus (100%), Bat (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ADRA1B antibody (ABIN6746726)

Mirski, Gerlach, Cole: "Sequence determinants of nuclear localization in the alpha and beta isoforms of human topoisomerase II." in: Experimental cell research, Vol. 251, Issue 2, pp. 329-39, (1999) (PubMed).

Brown, Holden, Rahn, Perkins: "Immunohistochemical staining for DNA topoisomerase IIa in Hodgkin's disease." in: American journal of clinical pathology, Vol. 109, Issue 1, pp. 39-44, (1998) (PubMed).

Herzog, Holmes, Tuschong, Ganapathi, Zwelling: "Absence of topoisomerase IIbeta in an amsacrine-resistant human leukemia cell line with mutant topoisomerase IIalpha." in: Cancer research, Vol. 58, Issue 23, pp. 5298-300, (1998) (PubMed).

Target Details for ADRA1B

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Alternative Name: ADRA1B

Background: Name/Gene ID: ADRA1B
Subfamily: Adrenergic
Family: GPCR

Synonyms: ADRA1B, Alpha 1b, Alpha-1B adrenergic receptor, Alpha-1b-adrenergic, Alpha-1B-adrenergic receptor, Alpha1b, Adrenoceptor alpha 1B, Alpha-1B adrenoreceptor, Alpha1B-AR, Alpha1beta-AR, ALPHA1BAR, ADRA1, Alpha-1B adrenoceptor, Alpha1b-adrenergic

Gene ID: 147

NCBI Accession: NP_000670

UniProt: P35368

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process