ADRA1B antibody (AA 388-437)

Catalog No. ABIN6746726

The Rabbit Polyclonal anti-ADRA1B antibody has been validated for WB. It is suitable to detect ADRA1B in samples from Human, Mouse, Rat, Dog, Cow, Pig, Monkey, Guinea Pig, Rabbit and Hamster. There are 3+ publications available.

Quick Overview for ADRA1B antibody (AA 388-437) (ABIN6746726)

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Monkey, Guinea Pig, Rabbit, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRA1B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADRA1B Antibody

Binding Specificity: AA 388-437

Specificity: Human ADRA1B

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Bovine, Rabbit, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa388-437 of human ADRA1B (P35368, NP_000670). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Pig, Opossum, Guinea pig, Platypus (100%), Bat (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ADRA1B antibody (ABIN6746726)

Mirski, Gerlach, Cole: "Sequence determinants of nuclear localization in the alpha and beta isoforms of human topoisomerase II." in: Experimental cell research, Vol. 251, Issue 2, pp. 329-39, (1999) (PubMed).

Brown, Holden, Rahn, Perkins: "Immunohistochemical staining for DNA topoisomerase IIa in Hodgkin's disease." in: American journal of clinical pathology, Vol. 109, Issue 1, pp. 39-44, (1998) (PubMed).

Herzog, Holmes, Tuschong, Ganapathi, Zwelling: "Absence of topoisomerase IIbeta in an amsacrine-resistant human leukemia cell line with mutant topoisomerase IIalpha." in: Cancer research, Vol. 58, Issue 23, pp. 5298-300, (1998) (PubMed).

Target Details for ADRA1B

Target: ADRA1B (Adrenergic, alpha-1B-, Receptor (ADRA1B))

Alternative Name: ADRA1B

Background: Name/Gene ID: ADRA1B
Subfamily: Adrenergic
Family: GPCR

Synonyms: ADRA1B, Alpha 1b, Alpha-1B adrenergic receptor, Alpha-1b-adrenergic, Alpha-1B-adrenergic receptor, Alpha1b, Adrenoceptor alpha 1B, Alpha-1B adrenoreceptor, Alpha1B-AR, Alpha1beta-AR, ALPHA1BAR, ADRA1, Alpha-1B adrenoceptor, Alpha1b-adrenergic

Gene ID: 147

NCBI Accession: NP_000670

UniProt: P35368

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process