ASNA1 antibody (AA 218-267)

Catalog No. ABIN6746776

The Rabbit Polyclonal anti-ASNA1 antibody has been validated for WB. It is suitable to detect ASNA1 in samples from Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit, Hamster, Monkey, Pig and Xenopus laevis. There are 4+ publications available.

Quick Overview for ASNA1 antibody (AA 218-267) (ABIN6746776)

Target: ASNA1 (ArsA Arsenite Transporter, ATP-Binding, Homolog 1 (ASNA1))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit, Hamster, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASNA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ASNA1 Antibody

Binding Specificity: AA 218-267

Specificity: Human ASNA1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa218-267 of human ASNA1 (O43681, NP_004308). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus, Stickleback, Zebrafish (100%), Drosophila (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ASNA1 antibody (ABIN6746776)

Ordentlich, Downes, Xie, Genin, Spinner, Evans: "Unique forms of human and mouse nuclear receptor corepressor SMRT." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 2639-44, (1999) (PubMed).

Wagner, Norris, Knotts, Weigel, McDonnell: "The nuclear corepressors NCoR and SMRT are key regulators of both ligand- and 8-bromo-cyclic AMP-dependent transcriptional activity of the human progesterone receptor." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1369-78, (1998) (PubMed).

Nagy, Kao, Chakravarti, Lin, Hassig, Ayer, Schreiber, Evans: "Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase." in: Cell, Vol. 89, Issue 3, pp. 373-80, (1997) (PubMed).

Chen, Evans: "A transcriptional co-repressor that interacts with nuclear hormone receptors." in: Nature, Vol. 377, Issue 6548, pp. 454-7, (1995) (PubMed).

Target Details for ASNA1

Target: ASNA1 (ArsA Arsenite Transporter, ATP-Binding, Homolog 1 (ASNA1))

Alternative Name: ASNA1

Background: Name/Gene ID: ASNA1
Subfamily: Arsenite resistance efflux pump
Family: Transporter

Synonyms: ASNA1, Arsenical pump-driving ATPase, ASNA-I, ARSA-I, ARSA1, Arsenite-stimulated ATPase, ATPase ASNA1, Golgi to ER traffic 3 homolog, HARSA-1, GET3, HASNA-1, HASNA-I, TRC40, Arsenite-translocating ATPase, HARSA-I

Gene ID: 439

NCBI Accession: NP_004308

UniProt: O43681

Pathways: Positive Regulation of Peptide Hormone Secretion