Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody

Antigen: Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1)

Synonyms: B1, S7, Bp35, CD20, MS4A2, LEU-16, MGC3969, Cd20, Ly-44, Ms4a2, AA960661, FcRB, Fce1b, Ms4a1, Fcer1b, Fcrbeta, FCIGA, MS4A1, bp35, cd20, ms4a2, leu-16, ms4a4c, cd20-like

Clonality: Monoclonal (2H7)

Host: Mouse

Reactivity: Human, Primate

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN611632

Additional Information

Alternative name: CD20

Gene ID: 931

Immunogen: Human tonsillar B cells

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 2H7

Specificity: The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

Application Details

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Cancer, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Deans, Kalt, Ledbetter et al.: "Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20." in: The Journal of biological chemistry, Vol. 270, Issue 38, pp. 22632-8, 1995 (PubMed).

Deans, Robbins, Polyak et al.: "Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 344-8, 1998 (PubMed).

Polyak, Deans: "Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure." in: Blood, Vol. 99, Issue 9, pp. 3256-62, 2002 (PubMed).

Rose, Smith, Maloney: "Glucocorticoids and rituximab in vitro: synergistic direct antiproliferative and apoptotic effects." in: Blood, Vol. 100, Issue 5, pp. 1765-73, 2002 (PubMed).

Polyak, Ayer, Szczepek et al.: "A cholesterol-dependent CD20 epitope detected by the FMC7 antibody." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 17, Issue 7, pp. 1384-9, 2003 (PubMed).

Chan, Hughes, French et al.: "CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts." in: Cancer research, Vol. 63, Issue 17, pp. 5480-9, 2003 (PubMed).

Diehl, Schmidlin, Nagasawa et al.: "STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 4805-15, 2008 (PubMed).

Hayden-Ledbetter, Cerveny, Espling et al.: "CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 8, pp. 2739-46, 2009 (PubMed).