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Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) Antibody (DyLight 633)

Details for Product No. ABIN611866, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4371
1724
1660
896
804
618
560
279
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Sheep (Ovine)
2652
2596
2291
1654
1388
733
606
416
348
336
332
325
306
259
165
148
118
112
40
27
21
21
12
8
7
5
5
5
4
4
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
Host
Donkey
6509
4552
1743
764
646
367
93
51
50
8
8
5
4
3
1
1
Conjugate
DyLight 633
2122
1812
1684
1295
720
633
514
301
283
282
271
264
212
186
178
117
81
79
69
54
49
39
35
25
23
21
19
18
15
15
15
15
14
14
14
14
13
11
10
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified Sheep IgG, whole molecule
Characteristics Donkey anti-Sheep IgG (H&L) - Affinity Pure, DyLight 633 Conjugate.
Fluorphore: DyLight 633 (Ex = 638 nm, Em = 658 nm).
Fluor Protein Ratio: Moles DyLight 633 per Mole Antibody.
Purification Affinity purified using solid phase bovine IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Kudla, Montessuit, Eskes, Berrier, Martinou, Ghazi, Antonsson: "The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22713-8, 2000 (PubMed).

Zhuang, Demirs, Kochevar: "p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 25939-48, 2000 (PubMed).

Background publications Wang, Yin, Chao, Milliman, Korsmeyer: "BID: a novel BH3 domain-only death agonist." in: Genes & development, Vol. 10, Issue 22, pp. 2859-69, 1996 (PubMed).