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Goat anti-Human IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN612018, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4586
1866
1790
962
821
643
618
279
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
2753
2709
2346
1737
1452
749
638
448
383
356
356
349
315
263
165
150
130
113
41
27
21
21
12
8
7
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
Host
Goat
6859
4762
1779
768
674
368
92
52
51
8
8
5
4
3
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified human IgG, whole molecule
Characteristics Goat anti-human IgG (H&L) - Affinity Pure, DyLight 650 Conjugate.
Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm).
Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody.
Purification Affinity purified using solid phase human IgE (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Aguilar, Boehm, Gorshkova, Crouch, Tomita, Saito, Ohno, Bonifacino: "Signal-binding specificity of the mu4 subunit of the adaptor protein complex AP-4." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 13145-52, 2001 (PubMed).

DellAngelica, Mullins, Bonifacino: "AP-4, a novel protein complex related to clathrin adaptors." in: The Journal of biological chemistry, Vol. 274, Issue 11, pp. 7278-85, 1999 (PubMed).

Hirst, Bright, Rous, Robinson: "Characterization of a fourth adaptor-related protein complex." in: Molecular biology of the cell, Vol. 10, Issue 8, pp. 2787-802, 1999 (PubMed).