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Details for Product No. ABIN612393

Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody

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Antigen
Epitope
»Alternatives Heavy & Light Chain
Reactivity
»Alternatives Rabbit
Host
Conjugate
»Alternatives Un-conjugated
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
Pubmed 3 references available
Catalog no. ABIN612393
Quantity 1 mg
Price
110.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen Purified rabbit IgG, whole molecule
Purification Affinity purified using solid phase human IgM (H&L)
Purity Affinity purified antibody is > 95% based on SDS-PAGE
Background Goat anti-rabbit IgG (H&L) - Affinity Pure, DyLight 550 Conjugate, min x w/human serum proteins. Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 550 (Ex = 550 nm, Em = 576 nm). Fluor Protein Ratio: Moles DyLight 550 per Mole Antibody.
Research Area Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications
Comment

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/ml (E 1% at 280 nm = 13.0)
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide
Preservative Sodium azide
Storage 4 °C
Product cited in: Dimmeler, Fleming, Fisslthaler et al.: "Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation." in: Nature, Vol. 399, Issue 6736, pp. 601-5, 1999 (PubMed).

Gallis, Corthals, Goodlett et al.: "Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30101-8, 1999 (PubMed).

Michell, Chen Zp, Tiganis et al.: "Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17625-8, 2001 (PubMed).

Alternatives for antigen "IgG", type "Secondary Antibodies"
Hosts (3908), (2738), (1027), (379), (209), (208), (29), (26), (14), (6), (3), (3), (2), (1), (1)
Reactivities (1575), (1385), (1281), (1023), (786), (406), (351), (229), (227), (204), (188), (184), (179), (175), (89), (76), (62), (53), (32), (31), (18), (12), (6), (5), (4), (3), (3), (1)
Conjugates (1311), (1201), (1020), (853), (582), (412), (367), (201), (199), (116), (115), (113), (111), (101), (84), (31), (27), (20), (20), (17), (15), (13), (13), (10), (10), (10), (10), (10), (10), (10), (7), (7), (6), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4077), (941), (683), (143), (67), (65), (40), (20), (14), (14), (14), (14), (4), (4), (2), (1)
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