Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN612393
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Antigen
Epitope
Heavy & Light Chain
(3850), (1816), (1476), (1036), (776), (228), (189), (73), (38), (33), (32), (7), (5), (2), (2), (2), (2), (1), (1)
Reactivity
Rabbit
(1937), (1909), (1582), (1238), (979), (498), (435), (343), (298), (266), (266), (260), (226), (222), (122), (93), (84), (81), (36), (28), (21), (14), (8), (8), (7), (5), (5), (4), (3), (3), (2), (1), (1), (1)
Host
Goat
(4895), (3556), (1210), (463), (420), (254), (67), (48), (25), (8), (5), (1)
Conjugate
Un-conjugated
(1541), (1379), (1250), (1003), (674), (553), (413), (218), (201), (197), (132), (128), (115), (111), (36), (34), (25), (21), (20), (15), (11), (10), (10), (10), (10), (10), (10), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
Pubmed 3 references available
Quantity 1 mg
Options
Shipping to United States (Change)
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Catalog No. ABIN612393
165.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified rabbit IgG, whole molecule
Characteristics Goat anti-rabbit IgG (H&L) - Affinity Pure, DyLight 550 Conjugate.
Fluorphore: DyLight 550 (Ex = 550 nm, Em = 576 nm).
Fluor Protein Ratio: Moles DyLight 550 per Mole Antibody.
Purification Affinity purified using solid phase human IgM (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Dimmeler, Fleming, Fisslthaler et al.: "Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation." in: Nature, Vol. 399, Issue 6736, pp. 601-5, 1999 (PubMed).

Gallis, Corthals, Goodlett et al.: "Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30101-8, 1999 (PubMed).

Michell, Chen Zp, Tiganis et al.: "Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17625-8, 2001 (PubMed).

Validation Images
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