SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (C-Term) antibody

Details for Product No. ABIN614772, Supplier: Log in to see
Antigen
  • SMARCA4
  • BAF190
  • BAF190A
  • BRG1
  • MRD16
  • RTPS2
  • SNF2
  • SNF2L4
  • SNF2LB
  • SWI2
  • hSNF2b
  • brg-1
  • Brg1
  • HP1-BP72
  • SNF2beta
  • SW1/SNF
  • b2b692Clo
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
  • SMARCA4
  • Smarca4
Alternatives
anti-Human SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 antibody for Western Blotting
Epitope
C-Term
15
6
5
4
3
3
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
98
67
48
5
3
3
3
1
1
1
1
Host
Mouse
62
24
9
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
74
23
20
18
17
15
12
9
4
3
3
3
1
Options
Supplier
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Supplier Product No.
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Immunogen Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1
Clone BRG-01
Isotype IgG2a
Specificity The antibody BRG-01 recognizes an epitope within C-terminal part of Human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Protein-A affinity chromatography
Purity > 95 % pure by SDS-PAGE
Alternative Name SMARCA4 / BRG1 (SMARCA4 Antibody Abstract)
Background Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.Synonyms: ATP-dependent helicase SMARCA4, BRG-1 protein, Brahma protein homolog 1, Mitotic growth and transcription activator, Probable global transcription activator SNF2L4, SNF2-beta, SNF2B, SNF2L4, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
Gene ID 6597
NCBI Accession NP_001122316
UniProt P51532
Pathways Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (C-Term) antibody (ABIN614772) anti-SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4) (C-Term) antibody
Background publications Griffin, Brennan, Magnuson: "The chromatin-remodeling enzyme BRG1 plays an essential role in primitive erythropoiesis and vascular development." in: Development (Cambridge, England), Vol. 135, Issue 3, pp. 493-500, 2008 (PubMed).

Napolitano, Cipollaro, Cascino, Melone, Giordano, Galderisi: "Brg1 chromatin remodeling factor is involved in cell growth arrest, apoptosis and senescence of rat mesenchymal stem cells." in: Journal of cell science, Vol. 120, Issue Pt 16, pp. 2904-11, 2007 (PubMed).

Ooi, Belyaev, Miyake, Wood, Buckley: "BRG1 chromatin remodeling activity is required for efficient chromatin binding by repressor element 1-silencing transcription factor (REST) and facilitates REST-mediated repression." in: The Journal of biological chemistry, Vol. 281, Issue 51, pp. 38974-80, 2006 (PubMed).

Eroglu, Wang, Tu, Sun, Mivechi: "Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 235, Issue 10, pp. 2722-35, 2006 (PubMed).

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