Actin antibody (pan)

Catalog No. ABIN614804

The Mouse Monoclonal anti-Actin antibody has been validated for WB, IHC (p) and IHC (fro). It is suitable to detect Actin in samples from Human, Rat, Mouse, Chicken, Rabbit, Dog, Cat and Primate.

Quick Overview for Actin antibody (pan) (ABIN614804)

Target: Actin

Reactivity: Human, Rat, Mouse, Chicken, Rabbit, Dog, Cat, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HHF35

Product Details anti-Actin Antibody

Binding Specificity: pan

Cross-Reactivity (Details): Species reactivity (tested):Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken.

Purification: Protein-A Affinity Chromatography

Immunogen: SDS extracted protein fraction of Human myocardium.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Actin

Target: Actin

Background: Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.