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Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3) (C-Term) antibody

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Synonyms TIH1, Tih1, si:dz182n13.5, zgc:92333
(12), (4), (2), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(29), (12), (11)
(15), (13), (1)
(1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(21), (19), (8), (1)
Pubmed 1 reference available
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Quantity 0.1 mg
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Immunogen synthetic peptide corresponding to the C Terminus of the protein sequence of Huuman PHLDA3 (NP_036528.1). Genename: PHLDA3
Specificity Recognizes PHLDA3 / TIH1
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Alternative Name PHLDA3 (PHLDA3 Antibody Abstract)
Background P53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.Synonyms: Pleckstrin homology-like domain family A member 3, TDAG51/Ipl homologue 1, TIH1
Gene ID 23612
NCBI Accession NP_036528
UniProt Q9Y5J5
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3) (C-Term) antibody (ABIN615985) HEK293 lysate (10 µg protein in RIPA buffer) overexpressing Human PHLDA3 with C-termi...
Background publications Frank, Mendelsohn, Ciccone et al.: "A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 10, Issue 12, pp. 1150-9, 2000 (PubMed).

Catalog No. ABIN615985
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