PEX14 antibody (N-Term, pHis6)

Catalog No. ABIN5326727

Product Details anti-PEX14 Antibody

Target: PEX14 (Peroxisomal Biogenesis Factor 14 (PEX14))

Binding Specificity: N-Term, pHis6

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEX14 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Arabidopsis thaliana, Magnaporthe oryzae

Characteristics: Expected / apparent Molecular Weight of the Antigene: 55.5 / 75-65 kDa (depending upon the gel system)

Purification: serum

Immunogen: Recombinant, soluble N-terminal domain of Arabidopsis thaliana Pex14p (Q9FE40, At5g62810) that mediates PEX5 and PEX19 binding. The transmembrane and coiled-coil domains of PEX14 were replaced with a dual StrepII-His6 tag.

Application Details

Application Notes: 1: 10 000 with standard ECL (WB)

Comment:

Antibodies will detect Pex14 protein in both light- and dark-grown seedlings grown on petri dishes and in rosette leaves of adult plants grown in soil.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for PEX14

Target: PEX14 (Peroxisomal Biogenesis Factor 14 (PEX14))

Alternative Name: Pex14p (PEX14 Products)

Synonyms: ATPEX14 antibody, MQB2.13 antibody, MQB2_13 antibody, PED2 antibody, PEROXISOME DEFECTIVE 2 antibody, peroxin 14 antibody, NAPP2 antibody, PBD13A antibody, Pex14p antibody, dJ734G22.2 antibody, R75137 antibody, peroxisomal biogenesis factor 14 antibody, peroxin 14 antibody, PEX14 antibody, Pex14 antibody

Background: AGI Code: At5g62810
Pex14p has a protein transporter activity and is involved in protein targeting into peroxisome. Submitted protein name: Genomic DNA, chromosome 5, P1 clone:MQB2

Molecular Weight: expected: 55.5 kDa, apparent: 75-65 kDa (depending upon the gel system)

UniProt: Q9FE40

Pathways: Maintenance of Protein Location