Goat anti-Human IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN619623

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Fc Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Cross-Reactivity: Sheep (Ovine), Mouse (Murine), Rabbit

Predicted Reactivity: human IgG Fc (two heavy chains with constant domains)

Characteristics: Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab) '2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
F(ab)'2 fragment

Purification: Affinity purification

Immunogen: Purified human IgG

Isotype: IgG

Application Details

Application Notes: 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Comment:

This antibody reacts with the heavy (gamma) chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins, or non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins or mouse and rabbit IgG based on immunoelectrophoresis.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

Storage Comment: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody