KRT8 antibody

Catalog No. ABIN626196

The Mouse Monoclonal anti-KRT8 antibody (Clone C-51) (ABIN626196) specifically detects KRT8 in WB, IHC, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for KRT8 antibody (ABIN626196)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: C-51

Product Details anti-KRT8 Antibody

Specificity: For frozen and paraffin embedded tissues the optimal dilutions have to be determined for use within each laboratory.

Purification: Purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (1:50), WB

Usage: In immunochemistry on cultured cells it strongly reacts with methanol/acetone fixed cells of epithelial origin. In frozen section this antibody reacts with all simple epithelia derived structures). In paraffin embedded tissues a FKFM-fixation (40 % formalin, acetic acid and distilled water 2:1:17 with 8.5 g NaCl/L) should be used. Methacarn and formalin fixatives produce less satisfactory results. A protease digestion is beneficial for the result. This antibody can also be used in Western blotting.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: KRT8 / CK8 / Cytokeratin 8

Background: Name/Gene ID: KRT8
Family: Keratin

Synonyms: KRT8, CARD2, CK-8, CYK8, Cytokeratin-8, CK8, K2C8, K8, KO, Keratin-8, Type-II keratin Kb8, Cytokeratin 8, Keratin 8

Gene ID: 3856

UniProt: P05787