Hexokinase 2 antibody (Middle Region)

Catalog No. ABIN632021

The Rabbit Polyclonal anti-Hexokinase 2 antibody is suitable to detect Hexokinase 2 in samples from Human. It has been validated for WB.

Quick Overview for Hexokinase 2 antibody (Middle Region) (ABIN632021)

Target: Hexokinase 2 (HK2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase 2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Hexokinase 2 Antibody

Binding Specificity: Middle Region

Specificity: Hexokinase 2 antibody was raised against the middle region of HK2

Purification: Affinity purified

Immunogen: Hexokinase 2 antibody was raised using the middle region of HK2 corresponding to a region with amino acids QRIKENKGEERLRSTIGVDGSVYKKHPHFAKRLHKTVRRLVPGCDVRFLR

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

Hexokinase 2 Blocking Peptide, (ABIN5613996), is also available for use as a blocking control in assays to test for specificity of this Hexokinase 2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of HK2 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: Hexokinase 2

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK2 (hexokinase 2) is the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this protein is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Molecular Weight: 102 kDa (MW of target protein)

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect