NCBP1 antibody (N-Term)

Catalog No. ABIN633218

The Rabbit Polyclonal anti-NCBP1 antibody is suitable to detect NCBP1 in samples from Human. It has been validated for WB.

Quick Overview for NCBP1 antibody (N-Term) (ABIN633218)

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCBP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NCBP1 Antibody

Binding Specificity: N-Term

Specificity: NCBP1 antibody was raised against the N terminal of NCBP1

Purification: Affinity purified

Immunogen: NCBP1 antibody was raised using the N terminal of NCBP1 corresponding to a region with amino acids SRRRHSDENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLE

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

NCBP1 Blocking Peptide, (ABIN5614927), is also available for use as a blocking control in assays to test for specificity of this NCBP1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of NCBP1 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for NCBP1

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Alternative Name: NCBP1

Background: NCBP1 is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.

Molecular Weight: 92 kDa (MW of target protein)

Pathways: Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process