Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (C-Term) antibody

Antigen: Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5)

Synonyms: BIP, MIF2, GRP78, FLJ26106, HSPA5, DKFZp459C013, BiP, GRP-78, BiP/grp78, hspa5, MGC52648

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651231

Additional Information

Alternative name: HSPA5

Gene ID: 3309

UniProt: NP_005338, P11021

Immunogen: This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 548-587 amino acids from the C-terminal region of human HSPA5.

Isotype: Ig

Description: Other names: HSPA5,GRP78,78 kDa glucose-regulated protein

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 548-587 amino acids from the C-terminal region of human HSPA5.

Molecular Weight: 72333 DA

Comments:

Background: When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

HSPA5 Antibody (C-term) (ABIN651231) western blot analysis in A375,A2058,Hela cell line lysates (35ug/lane). This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).

HSPA5 Antibody (C-term) (ABIN651231) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSPA5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HSPA5 Antibody (C-term) (ABIN651231) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Zhuang, Scolyer, Murali et al.: "Lactate dehydrogenase 5 expression in melanoma increases with disease progression and is associated with expression of Bcl-XL and Mcl-1, but not Bcl-2 proteins." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 23, Issue 1, pp. 45-53, 2010 (PubMed).

Zhao, Zhang, Tian et al.: "Proteomic analysis of cell lines expressing small hepatitis B surface antigen revealed decreased glucose-regulated protein 78 kDa expression in association with higher susceptibility to apoptosis." in: Journal of medical virology, Vol. 82, Issue 1, pp. 14-22, 2009 (PubMed).