N(alpha)-Acetyltransferase 50, NatE Catalytic Subunit (NAA50) (C-Term) antibody

Antigen: N(alpha)-Acetyltransferase 50, NatE Catalytic Subunit (NAA50)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651251

Additional Information

Alternative name: NAT13

Gene ID: 80218

UniProt: Q9GZZ1

Immunogen: This NAT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-155 amino acids from the C-terminal region of human NAT13.

Isotype: Ig

Description: Other names: N-acetyltransferase san homolog, hSAN, N-acetyltransferase 5, hNAT5, N-acetyltransferase 13,NAT5, MAK3, NAT13

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NAT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-155 amino acids from the C-terminal region of human NAT13.

Molecular Weight: 19398 DA

Comments:

Background: NAT13 is probable catalytic component of the ARD1A-NARG1 complex which displays alpha (N-terminal) acetyltransferase activity.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies

Restrictions: For Research Use only

Images

Western blot analysis of NAT13 Antibody (C-term) (ABIN651251) in K562 cell line lysates (35ug/lane).NAT13 (arrow) was detected using the purified Pab.

NAT13 Antibody (C-term) (ABIN651251) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Starheim, Gromyko, Evjenth et al.: "Knockdown of human N alpha-terminal acetyltransferase complex C leads to p53-dependent apoptosis and aberrant human Arl8b localization." in: Molecular and cellular biology, Vol. 29, Issue 13, pp. 3569-81, 2009 (PubMed).