Glucose 6-Phosphatase, Catalytic (G6PC) (Center) antibody

Antigen: Glucose 6-Phosphatase, Catalytic (G6PC)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651417

Additional Information

Alternative name: G6PC

Gene ID: 2538

UniProt: P35575

Immunogen: This G6PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-161 amino acids from the Central region of human G6PC.

Isotype: Ig

Description: Other names: Glucose-6-phosphatase alpha, G6Pase-alpha, Glucose-6-phosphatase, G-6-Pase, G6Pase,G6PT, G6PC

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This G6PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-161 amino acids from the Central region of human G6PC.

Molecular Weight: 40484 DA

Comments:

Background: Glucose-6-phosphatase is an integral membrane protein of the endoplasmic reticulum that catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate. It is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Defects in the enzyme cause glycogen storage disease type I.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism

Restrictions: For Research Use only

Images

Western blot analysis of G6PC Antibody (Center) (ABIN651417) in K562 cell line lysates (35ug/lane).G6PC (arrow) was detected using the purified Pab.

G6PC Antibody (Center) (ABIN651417) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

G6PC Antibody (Center) (ABIN651417) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Samuel, Beddow, Iwasaki et al.: "Fasting hyperglycemia is not associated with increased expression of PEPCK or G6Pc in patients with Type 2 Diabetes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 29, pp. 12121-6, 2009 (PubMed).

Tu, Bagnall, Duflou et al.: "Post-mortem pathologic and genetic studies in "dead in bed syndrome" cases in type 1 diabetes mellitus." in: Human pathology, Vol. 41, Issue 3, pp. 392-400, 2010 (PubMed).