G6PC antibody (N-Term)

Catalog No. ABIN2781770

This Rabbit Polyclonal antibody specifically detects G6PC in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig and Sheep.It has been mentioned in 1 publication

Quick Overview for G6PC antibody (N-Term) (ABIN2781770)

Target: G6PC (Glucose 6-Phosphatase, Catalytic (G6PC))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G6PC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-G6PC Antibody

Binding Specificity: N-Term

Purpose: G6PC Antibody - N-terminal region

Sequence: NLVFKWILFG QRPYWWVLDT DYYSNTSVPL IKQFPVTCET GPGSPSGHAM

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 93%, Rat: 93%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against G6PC. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human G6PC

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44224-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-G6PC antibody (ABIN2781770)

Iwasa, Kobayashi, Mifuji-Moroka, Hara, Miyachi, Sugimoto, Tanaka, Fujita, Gabazza, Takei: "Branched-chain amino acid supplementation reduces oxidative stress and prolongs survival in rats with advanced liver cirrhosis." in: PLoS ONE, Vol. 8, Issue 7, pp. e70309, (2013) (PubMed).

Target Details for G6PC

Target: G6PC (Glucose 6-Phosphatase, Catalytic (G6PC))

Alternative Name: G6PC

Background:

Background Information: G6PC hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum.It forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.Glucose-6-phosphatase is an integral membrane protein of the endoplasmic reticulum that catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate. It is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Defects in the enzyme cause glycogen storage disease type I (von Gierke disease). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Glucose-6-phosphatase, catalytic subunit

Alternative Symbols: G6PC, G6PT, GSD1, GSD1a, G6Pase

Protein Name: Glucose-6-phosphatase

Molecular Weight: 40 kDa

Gene ID: 2538

NCBI Accession: NP_000142

UniProt: P35575

Pathways: Carbohydrate Homeostasis, Cellular Glucan Metabolic Process