RAB11 Family Interacting Protein 2 (Class I) (RAB11FIP2) (Center) antibody

Antigen: RAB11 Family Interacting Protein 2 (Class I) (RAB11FIP2)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651666

Additional Information

Alternative name: RAB11FIP2

Gene ID: 22841

UniProt: NP_055719.1, Q7L804

Immunogen: This RAB11FIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-382 amino acids from the Central region of human RAB11FIP2.

Isotype: Ig

Description: Other names: RAB11FIP2,KIAA0941,Rab11 family-interacting protein 2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RAB11FIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-382 amino acids from the Central region of human RAB11FIP2.

Molecular Weight: 58279 DA

Comments:

Background: RAB11FIP2 is an adapter protein that plays a role in the secretory pathway. It is thought to be important for endosome recycling and receptor-mediated endocytosis. In endosome recycling, RAB11-FIP2 regulates vesicle transport from the endosomal recycling compartment (ERC) to the plasma membrane.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Structure, Protein Modifications, Chromatin, Signaling

Restrictions: For Research Use only

Images

RAB11FIP2 Antibody (Center) (ABIN651666) western blot analysis in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the RAB11FIP2 antibody detected the RAB11FIP2 protein (arrow).

RAB11FIP2 Antibody (Center) (ABIN651666) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RAB11FIP2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

RAB11FIP2 Antibody (Center) (ABIN651666) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Utley, Ducharme, Varthakavi et al.: "Respiratory syncytial virus uses a Vps4-independent budding mechanism controlled by Rab11-FIP2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 29, pp. 10209-14, 2008 (PubMed).

Wang, Edwards, Riley et al.: "Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity." in: Cell, Vol. 135, Issue 3, pp. 535-48, 2008 (PubMed).