Hyaluronan Binding Protein 2 (HABP2) (C-Term) antibody

Antigen: Hyaluronan Binding Protein 2 (HABP2)

Synonyms: FSAP, HABP, PHBP, HGFAL, Habp, HABP2, MGC128149, habp2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651853

Additional Information

Alternative name: HABP2

Gene ID: 3026

UniProt: NP_004123, Q14520

Immunogen: This HABP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-414 amino acids from the C-terminal region of human HABP2.

Isotype: Ig

Description: Other names: Hyaluronan-binding protein 2,HABP2,PHBP, HGFAL

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HABP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-414 amino acids from the C-terminal region of human HABP2.

Molecular Weight: 62672 DA

Comments:

Background: HABP2 is an extracellular serine protease that binds hyaluronic acid and is involved in cell adhesion. The encoded protein is synthesized as a single chain, but then undergoes an autoproteolytic event to form the functional heterodimer. Further autoproteolysis leads to smaller, inactive peptides. This protease is known to cleave urinary plasminogen activator, coagulation factor VII, and the alpha and beta chains of fibrinogen, but not prothrombin, plasminogen, or the gamma chain of fibrinogen. Two transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of HABP2 Antibody (C-term) (ABIN651853) in mouse liver tissue lysates (35ug/lane). HABP2 (arrow) was detected using the purified Pab.

HABP2 Antibody (C-term) (ABIN651853) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the HABP2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Gupta, Batchu, Datta: "Purification, partial characterization of rat kidney hyaluronic acid binding protein and its localization on the cell surface." in: European journal of cell biology, Vol. 56, Issue 1, pp. 58-67, 1992 (PubMed).