Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R) antibody

Antigen: Macrophage Stimulating 1 Receptor (C-Met-Related tyrosine Kinase) (MST1R)

Synonyms: RON, PTK8, CD136, CDw136, Fv2, Ron, STK, Fv-2, Stk, Ptk8, Cdw136

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652379

Additional Information

Alternative name: RON

Gene ID: 4486

UniProt: Q04912

Immunogen: This RON antibody is generated from rabbits immunized with RON recombinant protein.

Isotype: Ig

Description: Other names: p185-Ron, CDW136, CD136 antigen, MST1R, RON, Macrophage-stimulating protein receptor alpha chain, Macrophage-stimulating protein receptor beta chain, Macrophage-stimulating protein receptor MSPR, MSP receptor

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RON antibody is generated from rabbits immunized with RON recombinant protein between amino acids 163-514.

Molecular Weight: 152227 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of RON Antibody (ABIN652379) in HL-60 cell line lysates (35ug/lane). RON (arrow) was detected using the purified Pab.

Publications

Product: Zhou, He, Chen et al.: "Altered expression of the RON receptor tyrosine kinase in primary human colorectal adenocarcinomas: generation of different splicing RON variants and their oncogenic potential." in: Oncogene, Vol. 22, Issue 2, pp. 186-97, 2003 (PubMed).

Danilkovitch-Miagkova, Duh, Kuzmin et al.: "Hyaluronidase 2 negatively regulates RON receptor tyrosine kinase and mediates transformation of epithelial cells by jaagsiekte sheep retrovirus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 8, pp. 4580-5, 2003 (PubMed).

Penengo, Rubin, Yarden et al.: "c-Cbl is a critical modulator of the Ron tyrosine kinase receptor." in: Oncogene, Vol. 22, Issue 24, pp. 3669-79, 2003 (PubMed).