Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1) (C-Term) antibody

Antigen: Nucleotide-Binding Oligomerization Domain Containing 1 (NOD1)

Synonyms: CARD4, NLRC1, CLR7.1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652666

Additional Information

Alternative name: CARD4

Gene ID: 10392

UniProt: Q9Y239

Immunogen: This CARD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 924~953 amino acids from the C-terminal region of human CARD4.

Isotype: Ig

Description: Other names: Nucleotide-binding oligomerization domain-containing protein 1, Caspase recruitment domain-containing protein 4, CARD4, NOD1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CARD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 924~953 amino acids from the C-terminal region of human CARD4.

Molecular Weight: 107691 DA

Comments:

Background: CARD4 is a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of CARD4 Antibody (C-term) (ABIN652666) in HL-60 cell line lysates (35ug/lane). CARD4 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with CARD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

CARD4 Antibody (C-term) (ABIN652666) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Inohara, Koseki, del Peso et al.: "Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14560-7, 1999 (PubMed).

Inohara, Koseki, Lin et al.: "An induced proximity model for NF-kappa B activation in the Nod1/RICK and RIP signaling pathways." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27823-31, 2000 (PubMed).