Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1) (Center) antibody

Antigen: Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1)

Synonyms: CP7B, CBAS3, SPG5A, hct-1, AW261589, D3Ertd552e, CYP7B1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652732

Additional Information

Alternative name: CYP7B1

Gene ID: 9420

UniProt: O75881

Immunogen: This CYP7B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the Center region of human CYP7B1.

Isotype: Ig

Description: Other names: Cytochrome P450 7B1, 25-hydroxycholesterol 7-alpha-hydroxylase, Oxysterol 7-alpha-hydroxylase, CYP7B1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CYP7B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the Center region of human CYP7B1.

Molecular Weight: 58256 DA

Comments:

Background: CYP7B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CYP7B1 Antibody (Center) (ABIN652732) in Hela cell line lysates (35ug/lane). CYP7B1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CALR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

CYP7B1 Antibody (Center) (ABIN652732) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Schwarz, Lund, Russell: "Two 7 alpha-hydroxylase enzymes in bile acid biosynthesis." in: Current opinion in lipidology, Vol. 9, Issue 2, pp. 113-8, 1998 (PubMed).