Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1) (C-Term) antibody

Antigen: Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1)

Synonyms: VDR, CP2B, CYP1, PDDR, VDD1, VDDR, VDDRI, CYP27B, P450c1, CYP1alpha, Vdr, Cp2b, Cyp1, Pddr, Vdd1, Vddr, Cyp40, VddrI, Cyp27b, MGC91184, vdr, cp2b, cyp1, pddr, vdd1, vddr, vddri, cyp27b, p450c1, MGC88925, cyp1alpha

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN652999

Additional Information

Alternative name: CYP27B1

Gene ID: 1594

UniProt: O15528

Immunogen: This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479~508 amino acids from the C-terminal region of human CYP27B1.

Isotype: Ig

Description: Other names: Cytochrome P450VD1-alpha, Cytochrome P450C1 alpha, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, 25-OHD-1 alpha-hydroxylase, Calcidiol 1-monooxygenase, Cytochrome p450 27B1, Cytochrome P450 subfamily XXVIIB polypeptide 1, 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, CYP27B, CYP1ALPHA, CYP27B1

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479~508 amino acids from the C-terminal region of human CYP27B1.

Molecular Weight: 56504 DA

Comments:

Background: CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Synonyms: VDR, CP2B, CYP1, PDDR, VDD1, VDDR, VDDRI, CYP27B, P450c1, CYP1alpha, Vdr, Cp2b, Cyp1, Pddr, Vdd1, Vddr, Cyp40, VddrI, Cyp27b, MGC91184, vdr, cp2b, cyp1, pddr, vdd1, vddr, vddri, cyp27b, p450c1, MGC88925, cyp1alpha

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CYP27B1 Antibody (C-term) (ABIN652999) in mouse kidney tissue lysates (35ug/lane). CYP27B1 (arrow) was detected using the purified Pab.

CYP27B1 Antibody (C-term) (ABIN652999) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CYP27B1 Antibody (C-term) (ABIN652999)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP27B1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Simon, Munger, Xing Yang et al.: "Polymorphisms in vitamin D metabolism related genes and risk of multiple sclerosis." in: Multiple sclerosis (Houndmills, Basingstoke, England), Vol. 16, Issue 2, pp. 133-8, 2010 (PubMed).