MCM2 antibody (C-Term)

Catalog No. ABIN653324

The Rabbit Polyclonal anti-MCM2 antibody is suitable to detect MCM2 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for MCM2 antibody (C-Term) (ABIN653324)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB25168

Product Details anti-MCM2 Antibody

Binding Specificity: AA 649-677, C-Term

Predicted Reactivity: X

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human MCM2.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Alternative Name: MCM2

Background: MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Molecular Weight: 101896

Gene ID: 4171

NCBI Accession: NP_004517

UniProt: P49736

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA