T-Box 1 (TBX1) (C-Term) antibody

Antigen: T-Box 1 (TBX1)

Synonyms: DGS, TGA, CAFS, CTHM, DGCR, DORV, VCFS, TBX1C, MGC136724, zgc:136724, mp:zf637-3-000616, TBX1, dgs, tga, cafs, cthm, dgcr, dorv, vcfs, tbx1c, xtbx1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653331

Additional Information

Alternative name: TBX1

Gene ID: 6899

UniProt: O43435

Immunogen: This TBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334~364 amino acids from the C-terminal region of human TBX1.

Isotype: Ig

Description: Other names: Testis-specific T-box protein, T-box transcription factor TBX1, T-box protein 1, TBX1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334~364 amino acids from the C-terminal region of human TBX1.

Molecular Weight: 43133 DA

Comments:

Background: TBX1 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of TBX1 Antibody (C-term) (ABIN653331) in HepG2 cell line lysates (35ug/lane). TBX1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with TBX1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Yamagishi, Maeda, Hu et al.: "Tbx1 is regulated by tissue-specific forkhead proteins through a common Sonic hedgehog-responsive enhancer." in: Genes & development, Vol. 17, Issue 2, pp. 269-81, 2003 (PubMed).

Shalaby, Presneau, Idowu et al.: "Analysis of the fibroblastic growth factor receptor-RAS/RAF/MEK/ERK-ETS2/brachyury signalling pathway in chordomas." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 8, pp. 996-1005, 2009 (PubMed).

Beaujard, Chantot, Dubois et al.: "Atypical deletion of 22q11.2: detection using the FISH TBX1 probe and molecular characterization with high-density SNP arrays." in: European journal of medical genetics, Vol. 52, Issue 5, pp. 321-7, 2009 (PubMed).

Heike, Starr, Rieder et al.: "Single nucleotide polymorphism discovery in TBX1 in individuals with and without 22q11.2 deletion syndrome." in: Birth defects research. Part A, Clinical and molecular teratology, Vol. 88, Issue 1, pp. 54-63, 2010 (PubMed).