Hepcidin Antimicrobial Peptide (HAMP) (Center) antibody

Antigen: Hepcidin Antimicrobial Peptide (HAMP)

Synonyms: HAMP, HEPC, Hepcidin, DKFZp470L0111, PLTR, HFE2B, LEAP1, LEAP-1, HEPCIDIN, Hepc, Hamp1, Hepc1, hepcidin, MGC137038

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653413

Additional Information

Alternative name: HAMP

Gene ID: 57817

UniProt: P81172

Immunogen: This HAMP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24~54 amino acids from the Central region of human HAMP.

Isotype: Ig

Description: Other names: Hepcidin-20, Hepc20, Hepcidin-25, Hepc25, Putative liver tumor regressor, PLTR, Liver-expressed antimicrobial peptide 1, LEAP-1, Hepcidin, UNQ487/PRO1003, LEAP1, HEPC, HAMP

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HAMP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24~54 amino acids from the Central region of human HAMP.

Molecular Weight: 9408 DA

Comments:

Background: HAMP is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

Western blot analysis of HAMP Antibody (Center) (ABIN653413) in MDA-MB435 cell line lysates (35ug/lane). HAMP (arrow) was detected using the purified Pab.

Publications

Product: Hunter, Fulton, Ganz et al.: "The solution structure of human hepcidin, a peptide hormone with antimicrobial activity that is involved in iron uptake and hereditary hemochromatosis." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 37597-603, 2002 (PubMed).