Fanconi Anemia, Complementation Group C (FANCC) (C-Term) antibody

Antigen: Fanconi Anemia, Complementation Group C (FANCC)

Synonyms: Facc, BB116513, FANCC, zgc:154105, fancc, FA3, FAC, FACC, FLJ14675

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653475

Additional Information

Alternative name: FANCC

Gene ID: 2176

UniProt: Q00597

Immunogen: This FANCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529~558 amino acids from the C-terminal region of human FANCC.

Isotype: Ig

Description: Other names: Fanconi anemia group C protein, Protein FACC ,FACC, FAC, FANCC

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FANCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529~558 amino acids from the C-terminal region of human FANCC.

Molecular Weight: 63429 DA

Comments:

Background: The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity, they are related by their assembly into a common nuclear protein complex. This protein is for complementation group C.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology

Restrictions: For Research Use only

Images

FANCC Antibody (C-term) (ABIN653475) western blot analysis in HL-60,HepG2 cell line lysates (35ug/lane).This demonstrates the FANCC antibody detected the FANCC protein (arrow).

FANCC Antibody (C-term)(ABIN653475) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FANCC Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

FANCC Antibody (C-term) (ABIN653475) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Sinha, Singh, Alam et al.: "Alterations in candidate genes PHF2, FANCC, PTCH1 and XPA at chromosomal 9q22.3 region: pathological significance in early- and late-onset breast carcinoma." in: Molecular cancer, Vol. 7, pp. 84, 2009 (PubMed).

Palmieri, Wilson, Iversen et al.: "Polymorphism in the IL18 gene and epithelial ovarian cancer in non-Hispanic white women." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 17, Issue 12, pp. 3567-72, 2008 (PubMed).

Michiels, Laplanche, Boulet et al.: "Genetic polymorphisms in 85 DNA repair genes and bladder cancer risk." in: Carcinogenesis, Vol. 30, Issue 5, pp. 763-8, 2009 (PubMed).

McWilliams, Bamlet, de Andrade et al.: "Polymorphic variants in hereditary pancreatic cancer genes are not associated with pancreatic cancer risk." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 9, pp. 2549-52, 2009 (PubMed).