Bartter Syndrome, Infantile, with Sensorineural Deafness (Barttin) (BSND) (C-Term) antibody

Antigen: Bartter Syndrome, Infantile, with Sensorineural Deafness (Barttin) (BSND)

Synonyms: MGC47291, MGC93168, BSND, DKFZp469H092

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653815

Additional Information

Alternative name: BSND

Gene ID: 7809

UniProt: Q8WZ55

Immunogen: This BSND antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291~320 amino acids from the C-terminal region of human BSND.

Isotype: Ig

Description: Other names: Barttin, BART, BSND

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BSND antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291~320 amino acids from the C-terminal region of human BSND.

Molecular Weight: 35197 DA

Comments:

Background: BSND encodes an essential beta subunit for CLC chloride channels. These heteromeric channels localize to basolateral membranes of renal tubules and of potassium-secreting epithelia of the inner ear.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of BSND Antibody (C-term) (ABIN653815) in mouse liver tissue lysates (35ug/lane). BSND (arrow) was detected using the purified Pab.

BSND Antibody (C-term) (ABIN653815) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BSND Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Kathiresan, Willer, Peloso et al.: "Common variants at 30 loci contribute to polygenic dyslipidemia." in: Nature genetics, Vol. 41, Issue 1, pp. 56-65, 2008 (PubMed).

Riazuddin, Anwar, Fischer et al.: "Molecular basis of DFNB73: mutations of BSND can cause nonsyndromic deafness or Bartter syndrome." in: American journal of human genetics, Vol. 85, Issue 2, pp. 273-80, 2009 (PubMed).