Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3)

Synonyms: GLK, MAPKKKK3, RAB8IPL1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653817

Additional Information

Alternative name: MAP4K3

Gene ID: 8491

UniProt: Q8IVH8

Immunogen: This MAP4K3 antibody is generated from rabbits immunized with a recombinant protein of human MAP4K3.

Isotype: Ig

Description: Other names: Germinal center kinase-related protein kinase, GLK, MAPK/ERK kinase kinase kinase 3, MEK kinase kinase 3, MEKKK 3, Mitogen-activated protein kinase kinase kinase kinase 3,RAB8IPL1, MAP4K3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAP4K3 antibody is generated from rabbits immunized with a recombinant protein of human MAP4K3.

Molecular Weight: 101316 DA

Comments:

Background: MAP4K3 encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by protein affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of MAP4K3 Antibody (ABIN653817) in HepG2 cell line lysates (35ug/lane). MAP4K3 (arrow) was detected using the purified Pab.

MAP4K3 Antibody (ABIN653817) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAP4K3 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

MAP4K3 Antibody (ABIN653817) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Yan, Mieulet, Burgess et al.: "PP2A T61 epsilon is an inhibitor of MAP4K3 in nutrient signaling to mTOR." in: Molecular cell, Vol. 37, Issue 5, pp. 633-42, 2010 (PubMed).