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Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) antibody

Details for Product No. ABIN653817
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Antigen
Synonyms GLK, MAPKKKK3, MEKKK 3, MEKKK3, RAB8IPL1, 4833416M01Rik, 4833416M07Rik, 9530052P13Rik, Glk
Reactivity
Human
(40), (18), (11)
Host
Rabbit
(41), (6)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(46), (32), (19), (16), (7), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN653817
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Immunogen This MAP4K3 antibody is generated from rabbits immunized with a recombinant protein of human MAP4K3.
Clone RB22192
Isotype Ig
Specificity This MAP4K3 antibody is generated from rabbits immunized with a recombinant protein of human MAP4K3.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name MAP4K3
Background MAP4K3 encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).
Synonyms: Germinal center kinase-related protein kinase, GLK, MAPK/ERK kinase kinase kinase 3, MEK kinase kinase 3, MEKKK 3, Mitogen-activated protein kinase kinase kinase kinase 3,RAB8IPL1, MAP4K3
Pathways: MAPK signaling pathway
Molecular Weight 101316 Da
Gene ID 8491
UniProt Q8IVH8
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) antibody Western blot analysis of MAP4K3 Antibody (ABIN653817) in HepG2 cell line lysates (35 µg/lane). MAP4K3 (arrow) was detected using the purified polyclonal antibody.
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) antibody (2) MAP4K3 Antibody (ABIN653817) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) antibody (3) MAP4K3 Antibody (ABIN653817) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Yan, Mieulet, Burgess et al.: "PP2A T61 epsilon is an inhibitor of MAP4K3 in nutrient signaling to mTOR." in: Molecular cell, Vol. 37, Issue 5, pp. 633-42, 2010 (PubMed).

Validation Images
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