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MAF1 antibody (MAF1 Homolog (S. Cerevisiae)) (AA 90-117)

Details for Product anti-MAF1 Antibody No. ABIN654163, Supplier: Log in to see
Antigen
  • 1110068E11Rik
  • AU042856
  • maf1
Epitope
AA 90-117, Center
16
11
9
7
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
40
10
5
2
Host
Rabbit
37
4
Clonality (Clone)
Polyclonal ()
Conjugate
This MAF1 antibody is un-conjugated
4
4
4
2
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
33
21
19
14
5
4
2
1
1
1
1
Supplier
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Immunogen This MAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 AA from the Central region of human MAF1.
Clone RB24238
Isotype Ig
Specificity This MAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-125 amino acids from the Central region of human MAF1.
Predicted Reactivity Cow (Bovine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name MAF1 (MAF1 Antibody Abstract)
Background This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.
Synonyms: Repressor of RNA polymerase III transcription MAF1 homolog,MAF1,
Molecular Weight 28771 DA
Gene ID 84232
NCBI Accession NP_115648
UniProt Q9H063
Research Area Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Immunohistochemistry (IHC) image for anti-MAF1 antibody (MAF1 Homolog (S. Cerevisiae)) (AA 90-117) (ABIN654163) MAF1 antibody (Center) (ABIN654163) immunohistochemistry analysis in formalin fixed a...
Product cited in: Rohira, Chen, Allen et al.: "Covalent small ubiquitin-like modifier (SUMO) modification of Maf1 protein controls RNA polymerase III-dependent transcription repression." in: The Journal of biological chemistry, Vol. 288, Issue 26, pp. 19288-95, 2013 (PubMed).

Background publications Kantidakis, Ramsbottom, Birch et al.: "mTOR associates with TFIIIC, is found at tRNA and 5S rRNA genes, and targets their repressor Maf1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 26, pp. 11823-8, 2010 (PubMed).

Shor, Wu, Shakey et al.: "Requirement of the mTOR kinase for the regulation of Maf1 phosphorylation and control of RNA polymerase III-dependent transcription in cancer cells." in: The Journal of biological chemistry, Vol. 285, Issue 20, pp. 15380-92, 2010 (PubMed).

Lamesch, Li, Milstein et al.: "hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes." in: Genomics, Vol. 89, Issue 3, pp. 307-15, 2007 (PubMed).