Angiopoietin 1 (ANGPT1) (C-Term) antibody

Antigen: Angiopoietin 1 (ANGPT1)

Synonyms: AGP1, AGPT, ANG1, Ang1, Ang-1, 1110046O21Rik, Agpt, Agpt1, ang1, wu:fc76g06, ANGPT1, MGC143440, ANG-1, angpt1, MGC80788

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654409

Additional Information

Alternative name: ANGPT1

Gene ID: 284

UniProt: NP_001137.2, Q15389

Immunogen: This ANGPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-410 amino acids from the C-terminal region of human ANGPT1.

Isotype: Ig

Description: Other names: ANGPT1,Angiopoietin-1,KIAA0003

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ANGPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-410 amino acids from the C-terminal region of human ANGPT1.

Molecular Weight: 57513 DA

Comments:

Background: Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

ANGPT1 Antibody (C-term) (ABIN654409) western blot analysis in NCI-H292 cell line lysates (35ug/lane). This demonstrates the ANGPT1 antibody detected the ANGPT1 protein (arrow).

Publications

Product: Chen, Yang, Yu et al.: "A functional variant in the 3'-UTR of angiopoietin-1 might reduce stroke risk by interfering with the binding efficiency of microRNA 211." in: Human molecular genetics, Vol. 19, Issue 12, pp. 2524-33, 2010 (PubMed).

Fang, Neyrinck, Matthay et al.: "Allogeneic human mesenchymal stem cells restore epithelial protein permeability in cultured human alveolar type II cells by secretion of angiopoietin-1." in: The Journal of biological chemistry, Vol. 285, Issue 34, pp. 26211-22, 2010 (PubMed).

Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).