SEC13 Homolog (S. Cerevisiae) (SEC13) (Center) antibody

Antigen: SEC13 Homolog (S. Cerevisiae) (SEC13)

Synonyms: SEC13R, npp-20, SEC13L1, D3S1231E, Sec13r, Sec13l1, 1110003H02Rik, MGC105923

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654695

Additional Information

Alternative name: SEC13

Gene ID: 6396

UniProt: NP_899195.1, P55735

Immunogen: This SEC13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the Central region of human SEC13.

Isotype: Ig

Description: Other names: Protein SEC13 homolog,SEC13,D3S1231E, SEC13L1, SEC13R

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SEC13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the Central region of human SEC13.

Molecular Weight: 35541 DA

Comments:

Background: The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

SEC13 Antibody (Center) (ABIN654695) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the SEC13 antibody detected the SEC13 protein (arrow).

Publications

Product: Glavy, Krutchinsky, Cristea et al.: "Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160 subcomplex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 10, pp. 3811-6, 2007 (PubMed).

Hsia, Stavropoulos, Blobel et al.: "Architecture of a coat for the nuclear pore membrane." in: Cell, Vol. 131, Issue 7, pp. 1313-26, 2007 (PubMed).

Nielsen: "The coat protein complex II, COPII, protein Sec13 directly interacts with presenilin-1." in: Biochemical and biophysical research communications, Vol. 388, Issue 3, pp. 571-5, 2009 (PubMed).