SEC13 antibody (Center)

Catalog No. ABIN2854912

Product Details anti-SEC13 Antibody

Target: SEC13 (SEC13 Homolog (SEC13))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEC13 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Mouse (97 %), Rat (97 %), Xenopus laevis (88 %), Zebrafish (87 %), Bovine (96 %), Xenopus tropicalis (89 %)

Characteristics: Rabbit Polyclonal antibody to SEC13L1 (SEC13 homolog (S. cerevisiae))
SEC13L1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SEC13L1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-SEC13 antibody (ABIN2854912)

Wolfson, Chantranupong, Wyant, Gu, Orozco, Shen, Condon, Petri, Kedir, Scaria, Abu-Remaileh, Frankel, Sabatini: "KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1." in: Nature, Vol. 543, Issue 7645, pp. 438-442, (2017) (PubMed).

Target Details for SEC13

Target: SEC13 (SEC13 Homolog (SEC13))

Alternative Name: SEC13L1 (SEC13 Products)

Synonyms: D3S1231E antibody, SEC13L1 antibody, SEC13R antibody, npp-20 antibody, 1110003H02Rik antibody, Sec13l1 antibody, Sec13r antibody, Protein SEC13 homolog antibody, SEC13 homolog, nuclear pore and COPII coat complex component antibody, npp-20 antibody, SEC13 antibody, Sec13 antibody

Background: The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found.

Molecular Weight: 36 kDa

Gene ID: 6396