|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Mitogen-Activated Protein Kinase 8 (MAPK8) (Thr183, Tyr185) antibody
|Synonyms||JNK, JNK1, PRKM8, SAPK1, JNK1A2, JNK21B1/2, Prkm8, AI849689, Bsk, D-JNK, D-junk, DBSK/JNK, DJNK, DJNK/bsk, JNK/SAPK, Junk, SAPKa, dJUN, DmelCG5680, CG5680, T10F20.15|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This JNK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-199 amino acids from human JNK1.|
|Description||Other names: Mitogen-activated protein kinase 8,MAPK8,JNK1, PRKM8, SAPK1|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This JNK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-199 amino acids from human JNK1.|
|Molecular Weight||48296 DA|
Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Yang, Zheng, Cao et al.: "8-Bromo-7-methoxychrysin-induced apoptosis of hepatocellular carcinoma cells involves ROS and JNK." in: World journal of gastroenterology : WJG, Vol. 16, Issue 27, pp. 3385-93, 2010 (PubMed).
Dasgupta, Kar, Liu et al.: "Reactive oxygen species control senescence-associated matrix metalloproteinase-1 through c-Jun-N-terminal kinase." in: Journal of cellular physiology, Vol. 225, Issue 1, pp. 52-62, 2010 (PubMed).
Han, Crowe: "Jun amino-terminal kinase 1 activation promotes cell survival in ErbB2-positive breast cancer." in: Anticancer research, Vol. 30, Issue 9, pp. 3407-12, 2010 (PubMed).
|Hosts||Rabbit (61), Mouse (33), Sheep (2)|
|Reactivities||Human (88), Mouse (Murine) (23), Rat (Rattus) (16), Dog (Canine) (3), Caenorhabditis elegans (C. elegans) (2), Cow (Bovine) (2), Cat (Feline) (1), Chicken (1), Pig (Porcine) (1), Rabbit (1)|
|Applications||Western Blotting (WB) (82), ELISA (62), Immunoprecipitation (IP) (29), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (25), Immunohistochemistry (IHC) (21), Immunofluorescence (IF) (9), Dot Blot (Dot) (5), Immunocytochemistry (ICC) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Flow Cytometry (FACS) (2), Cellular Assay (CA) (1), Enzyme Immunoassay (EIA) (1), Functional Studies (Func) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1)|
|Epitopes||C-Term (8), pThr183, pTyr185 (6), Transcript Variant JNK1-A1 (5), pThr183 (4), pTyr185 (4), N-Term (3), Center (2), Ser63 (1), Thr183 (1), Thr183, Thr185 (1), Tyr185 (1), pTyr185,pThr183 (1), pTyr223,pThr221,pTyr185,pThr183 (1)|