Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5) (Center) antibody

Antigen: Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5)

Synonyms: ICH-3, MGC141966, ICEREL-III, ICE(rel)III, CASP5

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654898

Additional Information

Alternative name: CASP5

Gene ID: 838

UniProt: NP_001129582.1, P51878, NP_001129584.1, NP_004338.3, NP_001129581.1

Immunogen: This CASP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human CASP5.

Isotype: Ig

Description: Other names: Caspase-5,CASP5,ICH3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CASP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human CASP5.

Molecular Weight: 49736 DA

Comments:

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein, this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Eckhart, Kittel, Gawlas et al.: "Identification of a novel exon encoding the amino-terminus of the predominant caspase-5 variants." in: Biochemical and biophysical research communications, Vol. 348, Issue 2, pp. 682-8, 2006 (PubMed).

Ulybina, Kuligina, Mitiushkina et al.: "Evidence for depletion of CASP5 Ala90Thr heterozygous genotype in aged subjects." in: Experimental gerontology, Vol. 45, Issue 9, pp. 726-9, 2010 (PubMed).