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Nucleolar and Spindle Associated Protein 1 (NUSAP1) (AA 27-56), (N-Term) antibody

Details for Product No. ABIN654953, Supplier: Log in to see
Antigen
  • ANKT
  • BM037
  • LNP
  • NUSAP
  • PRO0310p1
  • Q0310
  • SAPL
  • YF-9
  • ankt
  • fb76a01
  • fi37a11
  • sb:cb490
  • wu:fb76a01
  • wu:fi37a11
  • lnp
  • nusap
  • nusap1
  • sapl
  • 2610201A12Rik
  • AI481307
  • AW547774
  • BB165529
  • NuSAP
Epitope
AA 27-56, N-Term
16
14
5
3
2
2
1
1
1
1
1
1
Reactivity
Human
60
22
18
1
1
1
1
1
Host
Rabbit
58
2
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
47
29
20
11
8
6
2
1
1
Supplier
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Immunogen This NUSAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 AA from the N-terminal region of human NUSAP.
Clone RB25410
Isotype Ig
Specificity This NUSAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human NUSAP.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NUSAP (NUSAP1 Antibody Abstract)
Background NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).
Synonyms: Nucleolar and spindle-associated protein 1,NUSAP1,ANKT
Molecular Weight 49452 DA
Gene ID 51203
NCBI Accession NP_060924, NP_057443, NP_001123369
UniProt Q9BXS6
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Nucleolar and Spindle Associated Protein 1 (NUSAP1) (AA 27-56), (N-Term) antibody (ABIN654953) NUSAP Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane)...
Immunohistochemistry (IHC) image for anti-Nucleolar and Spindle Associated Protein 1 (NUSAP1) (AA 27-56), (N-Term) antibody (ABIN654953) NUSAP antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffi...
Background publications Wadia, Coram, Armstrong et al.: "Antibodies specifically target AML antigen NuSAP1 after allogeneic bone marrow transplantation." in: Blood, Vol. 115, Issue 10, pp. 2077-87, 2010 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Raemaekers, Ribbeck, Beaudouin et al.: "NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization." in: The Journal of cell biology, Vol. 162, Issue 6, pp. 1017-29, 2003 (PubMed).