Asparaginyl-tRNA Synthetase (NARS) (AA 24-53), (N-Term) antibody

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Antigen
Epitope
AA 24-53, N-Term
(6), (5), (3), (3), (1), (1), (1), (1)
Reactivity
Human
(29), (4), (3)
Host
Rabbit
(17), (13)
Clonality (Clone)
Polyclonal ()
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(30), (11), (8), (7), (5), (4), (4), (3), (3), (3), (1), (1)
Pubmed 3 references available
Quantity 400 μL
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Immunogen This NARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 AA from the N-terminal region of human NARS.
Clone RB14847
Isotype Ig
Specificity This NARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-60 amino acids from the N-terminal region of human NARS.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Monkey
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name NARS (NARS Antibody Abstract)
Background Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.
Synonyms: Asparaginyl-tRNA synthetase, cytoplasmic,NARS,
Molecular Weight 62943 DA
Gene ID 4677
NCBI Accession NP_004530
UniProt O43776
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Asparaginyl-tRNA Synthetase (NARS) (AA 24-53), (N-Term) antibody Western blot analysis of NARS (arrow) using rabbit polyclonal NARS Antibody (N-term) ...
anti-Asparaginyl-tRNA Synthetase (NARS) (AA 24-53), (N-Term) antibody (2) NARS Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin e...
anti-Asparaginyl-tRNA Synthetase (NARS) (AA 24-53), (N-Term) antibody (3) NARS Antibody (N-term) western blot analysis in WiDr,HepG2 cell line lysates (35ug/l...
anti-Asparaginyl-tRNA Synthetase (NARS) (AA 24-53), (N-Term) antibody (4) NARS Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) com...
Background publications Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Beaulande, Tarbouriech, Härtlein: "Human cytosolic asparaginyl-tRNA synthetase: cDNA sequence, functional expression in Escherichia coli and characterization as human autoantigen." in: Nucleic acids research, Vol. 26, Issue 2, pp. 521-4, 1998 (PubMed).

Cirullo, Arredondo-Vega, Smith et al.: "Isolation and characterization of interspecific heat-resistant hybrids between a temperature-sensitive chinese hamster cell asparaginyl-tRNA synthetase mutant and normal human leukocytes: assignment of human asnS gene to chromosome 18." in: Somatic cell genetics, Vol. 9, Issue 2, pp. 215-33, 1983 (PubMed).

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Catalog No. ABIN655041
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