DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27) (C-Term) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27)

Synonyms: RHLP, Rrp3p, PP3241, HSPC259, MGC1018, FLJ12917, FLJ20596, FLJ22238, MGC163147, dJ686N3.1, DKFZp667N057, C86129, cb843, DDX27, MGC114699, rhlp, rrp3p, pp3241, hspc259

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655057

Additional Information

Alternative name: DDX27

Gene ID: 55661

UniProt: Q96GQ7, NP_060365.7

Immunogen: This DDX27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 777-796 amino acids from the C-terminal region of human DDX27.

Isotype: Ig

Description: Other names: Probable ATP-dependent RNA helicase DDX27,DDX27,RHLP

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DDX27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 777-796 amino acids from the C-terminal region of human DDX27.

Molecular Weight: 89835 DA

Comments:

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Chromatin, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

DDX27 Antibody (C-term) (ABIN655057) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).