DDX27 antibody

Catalog No. ABIN933004

This Mouse Monoclonal antibody specifically detects DDX27 in WB, FACS, DB and ICC. It exhibits reactivity toward Human.

Quick Overview for DDX27 antibody (ABIN933004)

Target: DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDX27 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB), Immunocytochemistry (ICC)

Clone: 2251C2a

Product Details anti-DDX27 Antibody

Cross-Reactivity: Human, Mouse (Murine)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: DDX27 antibody was raised in mouse using recombinant Human Dead (Asp-Glu-Ala-Asp) Box Polypeptide 27 (Ddx27)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: DDX27 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for DDX27

Target: DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))

Alternative Name: DDX27

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. Synonyms: Monoclonal DDX27 antibody, Anti-DDX27 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 antibody, RHLP antibody, Rrp3p antibody, PP3241 antibody, HSPC259 antibody, MGC1018 antibody, FLJ12917 antibody, FLJ20596 antibody, FLJ22238 antibody, dJ686N3.1 antibody, DKFZp667N057 antibody.