Deoxynucleotidyltransferase, terminal (DNTT) (C-Term) antibody

Antigen: Deoxynucleotidyltransferase, terminal (DNTT)

Synonyms: TDT, Tdt, BB160593, DNTT, MGC109152, dntt-A, TdT

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655508

Additional Information

Alternative name: TdT

Gene ID: 1791

UniProt: NP_001017520.1, NP_004079.3, P04053

Immunogen: This TdT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-475 amino acids from the C-terminal region of human TdT.

Isotype: Ig

Description: Other names: DNA nucleotidylexotransferase,DNTT,TDT

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TdT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-475 amino acids from the C-terminal region of human TdT.

Molecular Weight: 58536 DA

Comments:

Background: This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Signaling, Protein Modifications, Chromatin

Restrictions: For Research Use only

Images

TdT Antibody (C-term) (ABIN655508) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the TdT antibody detected the TdT protein (arrow).

Publications

Product: Liu, McGavran, Lovell et al.: "Nonpositive terminal deoxynucleotidyl transferase in pediatric precursor B-lymphoblastic leukemia." in: American journal of clinical pathology, Vol. 121, Issue 6, pp. 810-5, 2004 (PubMed).