2,3-bisphosphoglycerate Mutase (BPGM) (C-Term) antibody

Antigen: 2,3-bisphosphoglycerate Mutase (BPGM)

Synonyms: C86192, AI323730, AL022789, Ab2-098, zgc:92230, MGC127743

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656546

Additional Information

Alternative name: BPGM

Gene ID: 669

UniProt: P07738, NP_954655.1, NP_001715.1

Immunogen: This BPGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-259 amino acids from the C-terminal region of human BPGM.

Isotype: Ig

Description: Other names: Bisphosphoglycerate mutase,BPGM,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BPGM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-259 amino acids from the C-terminal region of human BPGM.

Molecular Weight: 30005 DA

Comments:

Background: 2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: BPGM Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Fujita, Suzuki, Tada et al.: "Human erythrocyte bisphosphoglycerate mutase: inactivation by glycation in vivo and in vitro." in: Journal of biochemistry, Vol. 124, Issue 6, pp. 1237-44, 1999 (PubMed).

Wang, Wei, Bian et al.: "Crystal structure of human bisphosphoglycerate mutase." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 39132-8, 2004 (PubMed).

Wang, Liu, Wei et al.: "Seeing the process of histidine phosphorylation in human bisphosphoglycerate mutase." in: The Journal of biological chemistry, Vol. 281, Issue 51, pp. 39642-8, 2006 (PubMed).

Lamesch, Li, Milstein et al.: "hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes." in: Genomics, Vol. 89, Issue 3, pp. 307-15, 2007 (PubMed).