Glutaryl-CoA Dehydrogenase (GCDH) (C-Term) antibody

Antigen: Glutaryl-CoA Dehydrogenase (GCDH)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656829

Additional Information

Alternative name: GCDH

Gene ID: 2639

UniProt: Q92947, NP_000150.1, NP_039663.1

Immunogen: This GCDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-365 amino acids from the C-terminal region of human GCDH.

Isotype: Ig

Description: Other names: Glutaryl-CoA dehydrogenase, mitochondrial,GCDH,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GCDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-365 amino acids from the C-terminal region of human GCDH.

Molecular Weight: 48127 DA

Comments:

Background: The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: GCDH Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

GCDH Antibody (C-term) (ABIN656829) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GCDH Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Ganesh, Zakai, van Rooij et al.: "Multiple loci influence erythrocyte phenotypes in the CHARGE Consortium." in: Nature genetics, Vol. 41, Issue 11, pp. 1191-8, 2009 (PubMed).