GCDH antibody (C-Term)

Catalog No. ABIN2781317

This Rabbit Polyclonal antibody specifically detects GCDH in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Rabbit, Dog, Horse and Pig. It has been mentioned in 2+ publications

Quick Overview for GCDH antibody (C-Term) (ABIN2781317)

Target: GCDH (Glutaryl-CoA Dehydrogenase (GCDH))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Rabbit, Dog, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GCDH Antibody

Binding Specificity: C-Term

Purpose: GCDH Antibody - C-terminal region

Sequence: IARQARDMLG GNGISDEYHV IRHAMNLEAV NTYEGTHDIH ALILGRAITG

Predicted Reactivity: Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against GCDH. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GCDH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8101657

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GCDH antibody (ABIN2781317)

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for GCDH

Target: GCDH (Glutaryl-CoA Dehydrogenase (GCDH))

Alternative Name: GCDH

Background:

Background Information: GCDH belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits.The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Gene Name: Glutaryl-CoA dehydrogenase

Alternative Symbols: GCD, ACAD5

Protein Name: Glutaryl-CoA dehydrogenase, mitochondrial

Molecular Weight: 48kDa

Gene ID: 2639

NCBI Accession: NP_000150

UniProt: Q92947