Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2) (C-Term) antibody

Antigen: Nuclear Factor (Erythroid-Derived 2), 45kDa (NFE2)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657484

Additional Information

Alternative name: NFE2

Gene ID: 4778

UniProt: Q16621, NP_001129495.1, NP_006154.1

Immunogen: This NFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-340 amino acids from the C-terminal region of human NFE2.

Isotype: Ig

Description: Other names: Transcription factor NF-E2 45 kDa subunit,NFE2,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-340 amino acids from the C-terminal region of human NFE2.

Molecular Weight: 41473 DA

Comments:

Background: Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: NFE2 Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

NFE2 Antibody (C-term) (ABIN657484) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the NFE2 antibody detected the NFE2 protein (arrow).

Publications

Product: Soranzo, Spector, Mangino et al.: "A genome-wide meta-analysis identifies 22 loci associated with eight hematological parameters in the HaemGen consortium." in: Nature genetics, Vol. 41, Issue 11, pp. 1182-90, 2009 (PubMed).

Motohashi, Kimura, Fujita et al.: "NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation." in: Blood, Vol. 115, Issue 3, pp. 677-86, 2010 (PubMed).

Lee, Shyu, Hsu et al.: "JNK-mediated turnover and stabilization of the transcription factor p45/NF-E2 during differentiation of murine erythroleukemia cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 1, pp. 52-7, 2010 (PubMed).

Zhou, Li, Deng et al.: "USF and NF-E2 cooperate to regulate the recruitment and activity of RNA polymerase II in the beta-globin gene locus." in: The Journal of biological chemistry, Vol. 285, Issue 21, pp. 15894-905, 2010 (PubMed).

Wang, Schwemmers, Hexner et al.: "AML1 is overexpressed in patients with myeloproliferative neoplasms and mediates JAK2V617F-independent overexpression of NF-E2." in: Blood, Vol. 116, Issue 2, pp. 254-66, 2010 (PubMed).